Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S98-7

Concentration

1 mg/ml

View all Neuroligin 4X/NLGN4X Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.

Localization

Cell Membrane , Cell Junction

Specificity

Approx 45% identity with Neuroligin-1, -2 and -3.Detects approx 25-130kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Neuroligin 4X/NLGN4X Antibody (S98-7)

Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]

Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]

Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198] - Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4X/NLGN4X protein using Mouse Anti-Neuroligin 4X/NLGN4X Monoclonal Antibody, Clone S98-7 (NBP2-42198). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4X/NLGN4X Monoclonal Antibody (NBP2-42198) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.
Immunocytochemistry/ Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]

Immunocytochemistry/ Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]

Immunocytochemistry/Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198] - Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehdye-fixed parrafin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH.

Applications for Neuroligin 4X/NLGN4X Antibody (S98-7)

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Neuroligin 4 Antibody was sufficient for detection of Neuroligin 4 in 20 ug of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuroligin 4X/NLGN4X

Neuroligin 4 X-Linked is expected to recognize both X-linked reported products (NP_065793 and NP_851849), which are identical, and the Y-linked reported product (NP_055708.2).

Alternate Names

ASPGX2, AUTSX2, HNL4X, HNLX, NL4, NLGN4, NLGN4X

Gene Symbol

NLGN4X

UniProt

B0F2B4 (Human)

Additional Neuroligin 4X/NLGN4X Products

Product Documents for Neuroligin 4X/NLGN4X Antibody (S98-7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neuroligin 4X/NLGN4X Antibody (S98-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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