Neurolysin Antibody (OTI1D6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01693
![Flow Cytometry: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Flow-Cytometry-NBP2-01693-img0001.jpg "Flow Cytometry: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey, Primate
Cited:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D6
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Neurolysin Antibody (OTI1D6)
Western Blot: NLN Antibody (1D6) [NBP2-01693] Analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
![Immunocytochemistry/ Immunofluorescence: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-01693-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
Immunocytochemistry/ Immunofluorescence: Neurolysin Antibody (OTI1D6) [NBP2-01693]
Immunocytochemistry/Immunofluorescence: NLN Antibody (1D6) [NBP2-01693] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN.![Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01693-img0006.jpg "Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]
Immunohistochemistry-Paraffin: NLN Antibody (1D6) [NBP2-01693] - Staining of paraffin-embedded Human tonsil using anti-NLN mouse monoclonal antibody.![Western Blot: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Western-Blot-NBP2-01693-img0002.jpg "Western Blot: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
![Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01693-img0003.jpg "Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
![Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01693-img0004.jpg "Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
![Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]](https://resources.bio-techne.com/images/products/NLN-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01693-img0005.jpg "Immunohistochemistry-Paraffin: Neurolysin Antibody (OTI1D6) [NBP2-01693]")
Applications for Neurolysin Antibody (OTI1D6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Neurolysin
Alternate Names
AGTBP, Neurotensin Endopeptidase, NLN, Oligopeptidase M
Entrez Gene IDs
57486 (Human)
Gene Symbol
NLN
Additional Neurolysin Products
Product Documents for Neurolysin Antibody (OTI1D6)
Product Specific Notices for Neurolysin Antibody (OTI1D6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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