Neuron-specific beta-III Tubulin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1195A
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Product Specifications
Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Detection of beta-III Tubulin in A172 cells by Flow Cytometry
A172 cells were stained with Mouse Anti-Neuron-specific beta-III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
Detection of beta‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.
Eight-day differentiated Rat cortical stem cells was stained with Mouse Anti-beta -III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Entrez Gene IDs
10381 (Human)
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Documents for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Product Specific Notices for Neuron-specific beta-III Tubulin APC-conjugated Antibody
For research use only
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