Neuropilin-1 Antibody (042) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89197

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 042

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Neuropilin-1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Neuropilin-1 (Accession#: NP_001019799.1; Met1-Lys644).

Specificity

No cross-reactivity in ELISA with: Human NRP2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Neuropilin-1 Antibody (042) - Azide and BSA Free

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human placenta with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human heart with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).

Applications for Neuropilin-1 Antibody (042) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuropilin-1

Neuropilin-1 (also known as Npn-1, NRP1, neuropilin, or CD304/BDCA4) is a 923 amino acid (aa) type I transmembrane glycoprotein (theoretical molecular weight 130-140 kDa) located on human chromosome 10p11.22 that encodes multiple intact and soluble isoforms. The 623 aa extracellular domain (ECD) of this human cell surface receptor includes two CUB (complement-binding) domains, two F5/8 domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. The ECD of human Neuropilin-1 shares 92-95% aa sequence identity with mouse, rat, bovine, and canine Npn-1. The MAM domains of neuropilins are involved in the formation of homo- and hetero-oligomers in the absence of ligands. Neuropilin-1 expression is found in neuronal, endothelial and tumor cells as well as epithelial cells such as in the respiratory, gastrointestinal, lower urological tracts, thyroid, parathyroid and thymus gland (1,2).
Initially found to play an essential role in axon growth and guidance, NRP1 serves as a multifunctional co-receptor for class III semaphorins and heparin-binding members of the VEGF family, participating in regulation of angiogenesis, neuronal development, cell survival, migration and tumor-invasion. Neuropilin-1 has been implicated in viral infections and T-cell activation and is also described as a marker of regulatory T (Treg) cells (3). In COVID-19 infections, studies have shown NRP1 participates in viral infection via NRP1 neutralizing antibodies and binds the SARS-CoV-2 Spike protein, suggesting NRP1 may contribute to viral transport and viral internalization by endocytosis (4).

References

1. Wild JRL, Staton CA, Chapple K, Corfe BM. (2012) Neuropilins: Expression and Roles in the Epithelium. Int J Exp Pathol. 93(2):81-103. PMID: 22414290

2. Bielenberg DR, Pettaway CA, Takashima S, Klagsbrun M. (2006) Neuropilins in Neoplasms: Expression, Regulation, and Function. Exp Cell Res. 312(5):584-93. PMID: 16445911

3. Tordjman R, Lepelletier Y, Lemarchandel V, Cambot M, Gaulard P, Hermine O, Romeo P-H. (2002). A Neuronal Receptor, neuropilin-1, Is Essential for the Initiation of the Primary Immune Response. Nat Immunol. 3(5):477-82. PMID: 11953749

4. Coronavirus research updates: Test frequency matters more than test sensitivity for stopping outbreaks. 2002 June 26. Nature.com

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Gene Symbol

NRP1

Additional Neuropilin-1 Products

Product Documents for Neuropilin-1 Antibody (042) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neuropilin-1 Antibody (042) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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