Neuropilin-1 Antibody (211H6.01)

Novus Biologicals, part of Bio-Techne | Catalog # DDX0440P-100

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 211H6.01

Concentration

0.5 mg/ml

View all Neuropilin-1 Antibodies »

Product Specifications

Immunogen

Neuropilin-1 Antibody (211H6.01) was made to Neuropilin/CD304.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neuropilin-1 Antibody (211H6.01)

Western Blot: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Western Blot: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Western Blot: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - IP of I^125-labeled mono-DC with Neuropilin-1 antibody (211H6.01) [DDX0440P-100]. Theoretical molecular weight 103 kDa. Observed molecular weight ~100 kDa.
Immunohistochemistry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Immunohistochemistry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Immunohistochemistry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - IHC staining of human tonsil using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].
Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100]

Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - FACS analysis of human PBMC using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].
View 1 more images

Applications for Neuropilin-1 Antibody (211H6.01)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

Tris-NaCl (pH 8.0)

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neuropilin-1

Neuropilin-1 (also known as Npn-1, NRP1, neuropilin, or CD304/BDCA4) is a 923 amino acid (aa) type I transmembrane glycoprotein (theoretical molecular weight 130-140 kDa) located on human chromosome 10p11.22 that encodes multiple intact and soluble isoforms. The 623 aa extracellular domain (ECD) of this human cell surface receptor includes two CUB (complement-binding) domains, two F5/8 domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. The ECD of human Neuropilin-1 shares 92-95% aa sequence identity with mouse, rat, bovine, and canine Npn-1. The MAM domains of neuropilins are involved in the formation of homo- and hetero-oligomers in the absence of ligands. Neuropilin-1 expression is found in neuronal, endothelial and tumor cells as well as epithelial cells such as in the respiratory, gastrointestinal, lower urological tracts, thyroid, parathyroid and thymus gland (1,2).
Initially found to play an essential role in axon growth and guidance, NRP1 serves as a multifunctional co-receptor for class III semaphorins and heparin-binding members of the VEGF family, participating in regulation of angiogenesis, neuronal development, cell survival, migration and tumor-invasion. Neuropilin-1 has been implicated in viral infections and T-cell activation and is also described as a marker of regulatory T (Treg) cells (3). In COVID-19 infections, studies have shown NRP1 participates in viral infection via NRP1 neutralizing antibodies and binds the SARS-CoV-2 Spike protein, suggesting NRP1 may contribute to viral transport and viral internalization by endocytosis (4).

References

1. Wild JRL, Staton CA, Chapple K, Corfe BM. (2012) Neuropilins: Expression and Roles in the Epithelium. Int J Exp Pathol. 93(2):81-103. PMID: 22414290

2. Bielenberg DR, Pettaway CA, Takashima S, Klagsbrun M. (2006) Neuropilins in Neoplasms: Expression, Regulation, and Function. Exp Cell Res. 312(5):584-93. PMID: 16445911

3. Tordjman R, Lepelletier Y, Lemarchandel V, Cambot M, Gaulard P, Hermine O, Romeo P-H. (2002). A Neuronal Receptor, neuropilin-1, Is Essential for the Initiation of the Primary Immune Response. Nat Immunol. 3(5):477-82. PMID: 11953749

4. Coronavirus research updates: Test frequency matters more than test sensitivity for stopping outbreaks. 2002 June 26. Nature.com

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Gene Symbol

NRP1

Additional Neuropilin-1 Products

Product Documents for Neuropilin-1 Antibody (211H6.01)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Neuropilin-1 Antibody (211H6.01)

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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