Neuroplastin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47357
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Neuroplastin Antibody
![Immunocytochemistry/ Immunofluorescence: Neuroplastin Antibody [NBP2-47357]](https://resources.bio-techne.com/images/products/Neuroplastin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47357-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Neuroplastin Antibody [NBP2-47357]")
Immunocytochemistry/ Immunofluorescence: Neuroplastin Antibody [NBP2-47357]
Immunocytochemistry/Immunofluorescence: Neuroplastin Antibody [NBP2-47357] - Staining of human cell line MCF7 shows localization to nucleoplasm, mitochondria & cytokinetic bridge.![Immunohistochemistry: Neuroplastin Antibody [NBP2-47357]](https://resources.bio-techne.com/images/products/Neuroplastin-Antibody-Immunohistochemistry-NBP2-47357-img0002.jpg "Immunohistochemistry: Neuroplastin Antibody [NBP2-47357]")
Immunohistochemistry: Neuroplastin Antibody [NBP2-47357]
Immunohistochemistry: Neuroplastin Antibody [NBP2-47357] - Staining of human cerebellum shows distinct positivity in cells in granular layer and neuropil. Purkinje cells were negative.
Western Blot: Neuroplastin Antibody [NBP2-47357] -
Western Blot: Neuroplastin Antibody [NBP2-47357] - Analysis in human cell line CAPAN-2.Applications for Neuroplastin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neuroplastin
Alternate Names
AW554172, GP55, GP66, NPTN, SDFR1, SDR1
Gene Symbol
NPTN
Additional Neuroplastin Products
Product Documents for Neuroplastin Antibody
Product Specific Notices for Neuroplastin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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