Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61658

Novus Biologicals, part of Bio-Techne
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NBP2-61658
NBP2-61658-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B6B10

Format

BSA Free

Concentration

1 mg/ml

View all Neutrophil Elastase/ELA2 Antibodies »

Product Summary for Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

Immunogen

Purified recombinant fragment of human Neutrophil Elastase/ELA2 (AA: 140-267) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10)BSA Free [NBP2-61658]

Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10)BSA Free [NBP2-61658]

Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free [NBP2-61658]

Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free [NBP2-61658]

Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free [NBP2-61658]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free [NBP2-61658]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).
View 3 more images

Applications for Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neutrophil Elastase/ELA2

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Neutrophil Elastase/ELA2 Antibody (6B6B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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