Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Human, Virus

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 6H12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Newcastle Disease Virus (NDV) Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Newcastle disease virus (La-sota strain).

Specificity

Specific to RNP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Newcastle Disease Virus (NDV) Antibody (6H12)

Immunocytochemistry/ Immunofluorescence: Newcastle Disease Virus (NDV) Antibody (6H12) [NBP2-11633]

Immunocytochemistry/ Immunofluorescence: Newcastle Disease Virus (NDV) Antibody (6H12) [NBP2-11633]

Immunocytochemistry/Immunofluorescence: Newcastle Disease Virus (NDV) Antibody (6H12) [NBP2-11633] - Representative fields of chicken neural cells infected with NDV TxGB strain, 12 hpi (MOI = 10). The same field (400x) and DAPI (pseudo-colored blue) were used in all images. 1st column shows cells stained for respective cellular markers. 2nd column shows NDV immunoreactivity in the same field. 3rd column (merged images of 1st & 2nd columns) shows colocalization of NDV & respective cellular markers. Double IFA signal (white arrows) shows cytoplasmic colocalization fluorescent signals of:Tuj-1 and NDV in scattered cells (c); nuclear (Olig-2) and cytoplasmic (NDV-BM) l in scattered cells (f); and cytoplasmic GFAP and NDV in scattered cells (i). Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-2053-z) licensed under a CC-BY license.

Applications for Newcastle Disease Virus (NDV) Antibody (6H12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in FLOW reported in scientific literature (PMID:31641164).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Newcastle Disease Virus (NDV)

Newcastle disease is a highly contagious bird disease affecting many domestic and wild avian species, most notably in domestic poultry. Newcastle disease is caused by Newcastle disease virus (NDV) a negative sense single stranded RNA virus. Transmission occurs by exposure to fecal and other excretions from infected birds. Exposure to humans can cause mild conjunctivitis and influenza like symptoms, but otherwise causes no hazard to health. NDV has the potential to be used as an anti-cancer agent. There are many different strains of NDV, and they have been classified as either lytic or nonlytic for human cells. Lytic strains and nonlytic strains both appear to replicate much more efficiently in human cancer cells than they do in most normal human cells, however, both classifications of NDV have the ability to selectively kill human tumor cells with limited toxicity to normal cells.

Alternate Names

NDV

Additional Newcastle Disease Virus (NDV) Products

Product Documents for Newcastle Disease Virus (NDV) Antibody (6H12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Newcastle Disease Virus (NDV) Antibody (6H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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