Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Mammal

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Cited:

In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NF-01

Format

BSA Free

Concentration

1.0 mg/ml

View all NF-H Antibodies »

Product Specifications

Immunogen

A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

Specificity

The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-H Antibody (NF-01) - BSA Free

Immunocytochemistry/ Immunofluorescence: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunocytochemistry/Immunofluorescence: NF-H Antibody (NF-01) [NB500-416] - Analysis using the Alexa Fluor (R) 488 conjugate of NB500-416. Ataining of NF-H in frozen monkey foot pad skin shows sensory fibers.Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) [NB500-416] - Human cerebellum (paraffin-embedded sections) with anti-200kDa Neurofilament Heavy protein (NF-01).
Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) - BSA Free [NB500-416]

Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) [NB500-416] - NF200 in rat cerebellum. Image submitted by a verified customer review.

Applications for NF-H Antibody (NF-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Purity > 95% (by SDS-PAGE). Use in In vitro reported in scientific literature (PMID 25993115). NF-H antibody validated for IHC-P, ICC/IF from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 5 using NB500-416 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NF-H

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. This antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32 enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, this antibody labels a neuronal population with enhanced vulnerability to kainate toxicity, most of which are GABAergic and reveal kainate activated Ca2+ uptake.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human); 380684 (Mouse); 24587 (Rat)

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for NF-H Antibody (NF-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NF-H Antibody (NF-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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