NF-H Antibody

Western Blot: NF-H Antibody [NB300-135]

Western Blot: NF-H Antibody [NB300-135] - Proof of viability of organotypic adult brain slices. Brain slices of adult 9 month old wildtype (WT) mice were dissected fresh or incubated for either 1 day or for 14 days to examine vitality of slices (A). Brain slices were subsequently analyzed by Western blot using antibodies against Neurofilament-200 kDa, total tau (Tau-5) and actin served as a control. Size markers are given as kDa on the right side. Note some degradation of neurofilament but stability of Tau-5. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fnagi.2018.00113/full) licensed under a CC-BY license.

Immunohistochemistry: NF-H Antibody [NB300-135]

Immunohistochemistry: NF-H Antibody [NB300-135] - Mouse hippocampus section stained with rabbit pAb to NF-H, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.

Immunohistochemistry: NF-H Antibody [NB300-135]

Immunohistochemistry: NF-H Antibody [NB300-135] - Immunostainings of adult organotypic brain slices at the hippocampal level. Brain slices were prepared from WT or TG adult mice and incubated with okadaic acid (OA). Brain slices were stained for nuclear DAPI (blue, A,B) showing intact brain structures. Thiazine Red staining (C,D) shows red fluorescent plaques in the hippocampus (D) of TG mice, while only background is seen in the hippocampus (C) of WT mice. Staining of neurofilament fibers (G,H) shows good neuronal viability both in WT and TG mice. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fnagi.2018.00113/full) licensed under a CC-BY license.