NF-L Antibody (NEFL/2983R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08553
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Canine, Chicken
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # NEFL/2983R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human NF-L protein (Uniprot: P07196)
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07407).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NF-L Antibody (NEFL/2983R) - Azide and BSA Free
Immunohistochemistry-Paraffin: NF-L Antibody (NEFL/2983R) - Azide and BSA Free [NBP3-08553]
Immunohistochemistry-Paraffin: NF-L Antibody (NEFL/2983R) - Azide and BSA Free [NBP3-08553] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-L Rabbit Monoclonal Antibody (NEFL/2983R).Applications for NF-L Antibody (NEFL/2983R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NF-L
Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Gene Symbol
NEFL
Additional NF-L Products
Product Documents for NF-L Antibody (NEFL/2983R) - Azide and BSA Free
Product Specific Notices for NF-L Antibody (NEFL/2983R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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