NF-L Antibody (NR-4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47969

Novus Biologicals, part of Bio-Techne
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NBP2-47969-0.1mg
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Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine, Chicken

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NR-4

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all NF-L Antibodies »

Product Specifications

Immunogen

Crude neurofilament preparation from porcine spinal cord

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44891).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NF-L Antibody (NR-4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Immunocytochemistry/ Immunofluorescence: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969] - Staining of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).
Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969] - Rat Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NR-4).
Flow Cytometry: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Flow Cytometry: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969]

Flow Cytometry: NF-L Antibody (NR-4) - Azide and BSA Free [NBP2-47969] - HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Applications for NF-L Antibody (NR-4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (NR-4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NF-L Antibody (NR-4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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