NF-L Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-131
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
Localization
Cytoplasmic
Specificity
Specifically recognizes the light neurofilament subunit (~68 kDa).
Marker
Neuronal Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NF-L Antibody
Western Blot: NF-L Antibody [NB300-131]
Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NB300-131]
Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. https://doi.org/10.1371/journal.pone.0192355Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NB300-131]
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNAApplications for NF-L Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:5000
Immunohistochemistry
1:5000
Immunohistochemistry-Paraffin
1:5000
Western Blot
1:10000-1:15000
Application Notes
This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Supplied as serum
Preservative
0.035% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF-L
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Gene Symbol
NEFL
UniProt
Additional NF-L Products
Product Specific Notices for NF-L Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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