NF2/Merlin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03740
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]](https://resources.bio-techne.com/images/products/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03740-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2/Merlin (NP_000259.1). TKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NF2/Merlin Antibody - BSA Free
Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] -
Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 180 s.
![Immunoprecipitation: NF2/Merlin Antibody - BSA Free [NBP3-03740]](https://resources.bio-techne.com/images/products/NF2-Merlin-Antibody-Immunoprecipitation-NBP3-03740-img0003.jpg "Immunoprecipitation: NF2/Merlin Antibody - BSA Free [NBP3-03740]")
Immunoprecipitation: NF2/Merlin Antibody - BSA Free [NBP3-03740]
Immunoprecipitation: NF2/Merlin Antibody [NBP3-03740] - Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2/Merlin antibody (NBP3-03740). Western blot was performed from the immunoprecipitate using NF2/Merlin antibody (NBP3-03740) at a dilution of 1:1000.
Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] -
Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of various lysates using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 30 s.
Applications for NF2/Merlin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NF2/Merlin
Long Name
Neurofibromin 2
Alternate Names
ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin
Gene Symbol
NF2
Additional NF2/Merlin Products
Product Documents for NF2/Merlin Antibody - BSA Free
Product Specific Notices for NF2/Merlin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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