NFAT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80257

Novus Biologicals, part of Bio-Techne
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NBP1-80257
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all NFAT5 Antibodies »

Product Summary for NFAT5 Antibody

Immunogen

Synthetic peptide directed towards the middle region of mouse NFAT5. Peptide sequence TIQTQDISQPGTFPAVSAASQLPSSDALLQQATQFQTREAQSRDTIQSDT. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for NFAT5 Antibody

Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257] - Host: Rabbit. Target Name: Nfat5. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 3ug/ml
Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257] - SP2/0 cell lysate, concentration 0.2-1 ug/ml.
Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257]

Western Blot: NFAT5 Antibody [NBP1-80257] - Lanes: Lane 1 : 20 ug HT 29 lysate blocked with 5% FBS Lane 2: 20 ug HT 29 lysate blocked with no FBS Lane 3: 20 ug HT-29 lyaste blocked with no PBS + 100 mM NaCl Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit-HRP Secondary, Antibody Dilution: 1 : 1000 Gene name: NFAT5.

Applications for NFAT5 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFAT5

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Alternate Names

EC 3.6.1, EC 3.6.3.14, KIAA0827glutamine rich protein H65, NF-AT5NFAT-like protein 1, NFATL1, NFATZ, nuclear factor of activated T-cells 5, tonicity-responsive, OREBP, osmotic response element-binding protein, T-cell transcription factor NFAT5, TonE-binding protein, TonEBP, TONEBPnuclear factor of activated T-cells 5, Tonicity-responsive enhancer-binding protein

Gene Symbol

NFAT5

UniProt

AAF79117 (Mouse)

Additional NFAT5 Products

Product Documents for NFAT5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFAT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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