NFATC1/NFAT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56732

Novus Biologicals, part of Bio-Techne
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NB100-56732
NB100-56732SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NFATC1 Antibodies »

Product Specifications

Immunogen

Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.

Specificity

The amino acid sequence used is 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC1/NFAT2 Antibody

Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]

Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]

Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732] - NFAT2 antibody was tested in Raw 246.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]

Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]

Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.
View 2 more images

Applications for NFATC1/NFAT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunohistochemistry

reported in scientific literature (PMID 35167499)

Simple Western

1:100

Western Blot

1-3 ug/ml
Application Notes
Immunoreative bands can be blocked by preincubation of NB100-56732 with immunizing peptide. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon testing material.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56732 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFATC1

NFAT (nuclear factor of activated T cell) transcription factors play critical roles in gene transcription during immune responses. The NFAT complex is made up of NFATc (NFATc1 - 4) and AP-1 transcription factor. NFAT proteins are regulated by Ca2+. NFATc1 is a substrate protein phosphatase calcineurin, a critical mediator of calcium signals during Tcell activation. NFATc1 activation requires the translocation of the transcription factor into the nucleus, a process that is mediated by calcineurin.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human)

Gene Symbol

NFATC1

UniProt

NP_006153.2 (Human)

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFATC1/NFAT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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