NFATC1/NFAT2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56732
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.
Specificity
The amino acid sequence used is 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFATC1/NFAT2 Antibody
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732]
Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732] - NFAT2 antibody was tested in Raw 246.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.Western Blot: NFATC1/NFAT2 Antibody [NB100-56732]
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.Applications for NFATC1/NFAT2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20
Immunohistochemistry
reported in scientific literature (PMID 35167499)
Simple Western
1:100
Western Blot
1-3 ug/ml
Application Notes
Immunoreative bands can be blocked by preincubation of NB100-56732 with immunizing peptide. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon testing material.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-56732 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFATC1
Long Name
Nuclear Factor of Activated T Cells C1
Alternate Names
NFAT2
Entrez Gene IDs
4772 (Human)
Gene Symbol
NFATC1
UniProt
Additional NFATC1 Products
Product Documents for NFATC1/NFAT2 Antibody
Product Specific Notices for NFATC1/NFAT2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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