NFATC2/NFAT1 Antibody (25A10.D6.D2) [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-504JF525

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB300-504AF350

Alexa Fluor 405

NB300-504AF405

Alexa Fluor 488

NB300-504AF488

Alexa Fluor 532

NB300-504AF532

Alexa Fluor 594

NB300-504AF594

Alexa Fluor 647

NB300-504AF647

Alexa Fluor 700

NB300-504AF700

Alexa Fluor 750

NB300-504AF750

Biotin

NB300-504B

CoraFluor 1

NB300-504CL1

DyLight 350

NB300-504UV

DyLight 405

NB300-504V

DyLight 488

NB300-504G

DyLight 550

NB300-504R

DyLight 594

NB300-504DL594

DyLight 650

NB300-504C

DyLight 680

NB300-504FR

DyLight 755

NB300-504IR

FITC

NB300-504F

HRP

NB300-504H

Janelia Fluor 549

NB300-504JF549

Janelia Fluor 585

NB300-504JF585

Janelia Fluor 635

NB300-504JF635

Janelia Fluor 646

NB300-504JF646

Janelia Fluor 669

NB300-504JF669

mFluor Violet 450 SE

NB300-504MFV450

mFluor Violet 500 SE

NB300-504MFV500

mFluor Violet 610 SE

NB300-504MFV610

Forumulation

Catalog #

BSA Free

NB300-504

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG₁ Clone # 25A10.D6.D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFATC2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for NFATC2/NFAT1 Antibody (25A10.D6.D2) [Janelia Fluor® 525]

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Gel Super Shift Assays

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NFATC2

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1, NFAT2, NFAT3, and NFAT4. These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Gene Symbol

NFATC2

Additional NFATC2 Products

Product Documents for NFATC2/NFAT1 Antibody (25A10.D6.D2) [Janelia Fluor® 525]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for NFATC2/NFAT1 Antibody (25A10.D6.D2) [Janelia Fluor® 525]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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