NFATC2/NFAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82583

Novus Biologicals, part of Bio-Techne
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NBP1-82583
NBP1-82583-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFATC2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DFSILFDYEYLNPNEEEPNAHKVASPPSGPAYPDDVLDYGLKPYSPLASLSGEPPGRFGEPDRVGPQKFLSAAKPAGASGLSPRIEITPSHELIQAVGPLRMRDAGLLVEQPPLAGVAASPRFTLPV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%). Mouse reactivity reported in scientific literature (PMID: 25719243).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC2/NFAT1 Antibody

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583]

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583]

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Analysis in human lymph node and skeletal muscle tissues. Corresponding NFATC2 RNA-seq data are presented for the same tissues.
Western Blot: NFATC2/NFAT1 Antibody [NBP1-82583]

Western Blot: NFATC2/NFAT1 Antibody [NBP1-82583]

Western Blot: NFATC2/NFAT1 Antibody [NBP1-82583] - Analysis in human cell line CACO-2.
Immunocytochemistry/ Immunofluorescence: NFATC2/NFAT1 Antibody [NBP1-82583]

Immunocytochemistry/ Immunofluorescence: NFATC2/NFAT1 Antibody [NBP1-82583]

Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody [NBP1-82583] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.
View 3 more images

Applications for NFATC2/NFAT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25719243). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFATC2

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1, NFAT2, NFAT3, and NFAT4. These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Gene Symbol

NFATC2

Additional NFATC2 Products

Product Documents for NFATC2/NFAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFATC2/NFAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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