NFATC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1082

Novus Biologicals, part of Bio-Techne
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NB100-1082
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NFATC4 Antibodies »

Product Summary for NFATC4 Antibody

Immunogen

Peptide with sequence C-SGFPAPPGEEPPA corresponding to C-Terminus according to NP_004545.2, NP_001185894.1, NP_001185895.1, NP_001185896.1.

Specificity

This antibody is expected to recognise isoform 2, 3, 4 and 5 (NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1 respectively).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NFATC4 Antibody

Immunohistochemistry-Paraffin: NFATC4 Antibody [NB100-1082]

Immunohistochemistry-Paraffin: NFATC4 Antibody [NB100-1082]

Immunohistochemistry-Paraffin: NFATC4 Antibody [NB100-1082] - 3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NFATC4 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, human brain and human heart lysates at upto 2ug/ml. IHC-P: Human placenta shows weak nuclear staining in trophoblasts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC4

The protein is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4.

Alternate Names

NF-AT3, NFAT3nuclear factor of activated T-cells, cytoplasmic 4, NFATc4, NF-ATc4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, T cell transcription factor NFAT3, T-cell transcription factor NFAT3

Entrez Gene IDs

4776 (Human)

Gene Symbol

NFATC4

UniProt

NP_004545 (Human)

Additional NFATC4 Products

Product Documents for NFATC4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFATC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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