NFIB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75588

Novus Biologicals, part of Bio-Techne
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NBP2-75588
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NFIB Antibodies »

Product Summary for NFIB Antibody

Immunogen

Recombinant protein within Human NFIB aa 1-273 / 420. (SwissProt: O00712 Human; SwissProt: P97863 Mouse)

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFIB Antibody

Western Blot: NFIB Antibody [NBP2-75588]

Western Blot: NFIB Antibody [NBP2-75588]

Western Blot: NFIB Antibody [NBP2-75588] - Analysis of NFIB/NF1B2 on PC-12 cell lysate using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-75588]

Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-75588]

Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-75588] - Analysis of paraffin-embedded mouse brain tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Flow Cytometry: NFIB Antibody [NBP2-75588]

Flow Cytometry: NFIB Antibody [NBP2-75588]

Flow Cytometry: NFIB Antibody [NBP2-75588] - Analysis of Hela cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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Applications for NFIB Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:200-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFIB

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein

Gene Symbol

NFIB

Additional NFIB Products

Product Documents for NFIB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFIB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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