NFIC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71878

Novus Biologicals, part of Bio-Techne
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NBP1-71878
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NFIC Antibodies »

Product Summary for NFIC Antibody

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human Nuclear Factor I/C using the numbering given in entry EAW69324.1 (GeneID 4782).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIC Antibody

Western Blot: NFIC Antibody [NBP1-71878]

Western Blot: NFIC Antibody [NBP1-71878]

Western Blot: NFIC Antibody [NBP1-71878] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71879, which recognizes a downstream epitope
Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC left image used at a dilution of 1:200 (1ug/ml), right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml), right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Co

Applications for NFIC Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFIC

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-binding transcription factor, CTF5, CTFCCAAT-box-binding transcription factor, MGC20153, NF1-C, NFI, NF-I, NF-I/C, NFI-C, nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, nuclear factor I/C (CCAAT-binding transcription factor), TGGCA-binding protein

Gene Symbol

NFIC

UniProt

EAW69324.1 (Human)

Additional NFIC Products

Product Documents for NFIC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFIC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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