NFIX Antibody (3D2)
Novus Biologicals, part of Bio-Techne | Catalog # H00004784-M08
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NFIX (NP_002492.2, 291 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHPLPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIR
Specificity
Reacts with nuclear factor I/X (CCAAT-binding transcription factor).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NFIX Antibody (3D2)
![Western Blot: NFIX Antibody (3D2) [H00004784-M08]](https://resources.bio-techne.com/images/products/NFIX-Antibody-3D2-Western-Blot-H00004784-M08-img0004.jpg "Western Blot: NFIX Antibody (3D2) [H00004784-M08]")
Western Blot: NFIX Antibody (3D2) [H00004784-M08]
Western Blot: NFIX Antibody (3D2) [H00004784-M08] - NFIX monoclonal antibody (M08), clone 3D2. Western Blot analysis of NFIX expression in A-431.![Western Blot: NFIX Antibody (3D2) [H00004784-M08]](https://resources.bio-techne.com/images/products/NFIX-Antibody-3D2-Western-Blot-H00004784-M08-img0003.jpg "Western Blot: NFIX Antibody (3D2) [H00004784-M08]")
Western Blot: NFIX Antibody (3D2) [H00004784-M08]
Western Blot: NFIX Antibody (3D2) [H00004784-M08] - Analysis of NFIX expression in Jurkat (Cat # L017V1).![ELISA: NFIX Antibody (3D2) [H00004784-M08]](https://resources.bio-techne.com/images/products/NFIX-Antibody-3D2-ELISA-H00004784-M08-img0002.jpg "ELISA: NFIX Antibody (3D2) [H00004784-M08]")
ELISA: NFIX Antibody (3D2) [H00004784-M08]
ELISA: NFIX Antibody (3D2) [H00004784-M08] - Detection limit for recombinant GST tagged NFIX is 0.03 ng/ml as a capture antibody.Applications for NFIX Antibody (3D2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFIX
Alternate Names
CTF, NF1ACCAAT-box-binding transcription factor, NF1-X, NF-I/X, NFI-X, nuclear factor 1 X-type, Nuclear factor 1/X, Nuclear factor I/X, nuclear factor I/X (CCAAT-binding transcription factor), TGGCA-binding protein
Entrez Gene IDs
4784 (Human)
Gene Symbol
NFIX
UniProt
Additional NFIX Products
Product Documents for NFIX Antibody (3D2)
Product Specific Notices for NFIX Antibody (3D2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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