NFkB p100/p52 Antibody (S04-4D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14959

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14959-100ul
NBP3-14959-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-4D6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFkB p100/p52 Antibodies »

Product Summary for NFkB p100/p52 Antibody (S04-4D6)

Immunogen

Recombinant protein of human NFkB p100/p52 (Uniprot#: Q00653)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB p100/p52 Antibody (S04-4D6)

Western Blot: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959]

Western Blot: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959]

Western Blot: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959] - Western blot detection of NFkB p100/p52 in C6, 3T3, Hela cell lysates using NFkB p100/p52 Rabbit mAb (1:1000 diluted). Predicted band size: 97kDa. Observed band size: 100kDa.
Immunocytochemistry/ Immunofluorescence: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959]

Immunocytochemistry/ Immunofluorescence: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959]

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959] - Immunofluorescence of NFkB p100/p52 (green) in Hela cells using NFkB p100/p52 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for NFkB p100/p52 Antibody (S04-4D6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p100/p52

The NFkappaB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkappaB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkappaB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Alternate Names

DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Lyt10, LYT10LYT-10, nuclear factor NF-kappa-B p100 subunit, nuclear factor of kappa light chain gene enhancer in B-cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), Oncogene Lyt-10, p52

Gene Symbol

NFKB2

Additional NFkB p100/p52 Products

Product Documents for NFkB p100/p52 Antibody (S04-4D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFkB p100/p52 Antibody (S04-4D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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