NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81272

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-81272-0.2mg
NBP2-81272SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-26C-15

Format

Azide and BSA Free

Concentration

1 mg/ml

View all NFkB p100/p52 Antibodies »

Product Summary for NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

Immunogen

Peptide "IEVDLVTHSDPPR" derived from the transcription factor NFKB2 (Nuclear factor NF-kappa-B p100 subunit) conjugated to KLH.

Specificity

Recognises human NFKB2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free [NBP2-81272]

Immunocytochemistry/ Immunofluorescence: NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free [NBP2-81272]

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272] - Immunofluorescence staining of fixed HeLa cells with NFkB p100/p52 Antibody (SAIC-26C-15) [NBP2-81272]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of SAIC-26C-15 [NBP2-81272] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, most likely in the ER. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81272, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: NFkB p100/p52

The NFkappaB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkappaB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkappaB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Alternate Names

DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Lyt10, LYT10LYT-10, nuclear factor NF-kappa-B p100 subunit, nuclear factor of kappa light chain gene enhancer in B-cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), Oncogene Lyt-10, p52

Gene Symbol

NFKB2

Additional NFkB p100/p52 Products

Product Documents for NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFkB p100/p52 Antibody (SAIC-26C-15) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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