NFS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54685

Novus Biologicals, part of Bio-Techne
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NBP1-54685
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NFS1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to NFS1 (NFS1 nitrogen fixation 1 homolog (S. cerevisiae)) The peptide sequence was selected from the middle region of NFS1. Peptide sequence TTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLV. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33673707).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for NFS1 Antibody

Western Blot: NFS1 Antibody [NBP1-54685]

Western Blot: NFS1 Antibody [NBP1-54685]

Western Blot: NFS1 Antibody [NBP1-54685] - Jurkat cell lysate, concentration 0.3125ug/ml.

Applications for NFS1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFS1

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. Two transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

cysteine desulfurase, mitochondrial, EC 2.8.1.7, NFS1 nitrogen fixation 1 homolog (S. cerevisiae), NifS, NIFShomolog), nitrogen-fixing bacteria S-like protein

Gene Symbol

NFS1

UniProt

Q9Y697 (Human)

Additional NFS1 Products

Product Documents for NFS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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