NFYA Antibody (SR1622)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22339

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22339-100ul
NBP3-22339-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1622

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFYA Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human NFYA (Uniprot #: P23511)

Specificity

Detects endogenous levels of total NFYA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFYA Antibody (SR1622)

Western Blot: NFYA Antibody (SR1622) [NBP3-22339] -

Western Blot: NFYA Antibody (SR1622) [NBP3-22339] - Western blot analysis using NBP3-22339 in 293T cell lysate.

Applications for NFYA Antibody (SR1622)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFYA

The Y box is a CCAAT box which is bound by the heteromeric DNA binding protein, NFY (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1 which also bind CCAAT like sequences, NFY exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30% of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter proximal sequences. NF-Y is composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.

Alternate Names

CAAT box DNA-binding protein subunit A, CBF-A, CBF-B, CCAAT-binding transcription factor subunit B, FLJ11236, HAP2, HAP2 CCAAT-binding protein, NF-YACAAT-box DNA binding protein subunit A, Nuclear transcription factor Y subunit A, nuclear transcription factor Y subunit alpha, nuclear transcription factor Y, alpha, Transcription factor NF-Y, A subunit

Gene Symbol

NFYA

Additional NFYA Products

Product Documents for NFYA Antibody (SR1622)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFYA Antibody (SR1622)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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