NG2/MCSP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89682
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ILYEHEMPPEPFWEAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NG2/MCSP Antibody
Immunohistochemistry: NG2/MCSP Antibody [NBP1-89682]
Immunohistochemistry: NG2/MCSP Antibody [NBP1-89682] - NG2/CSPG4 immunohistochemistry in oligodendroglial tumors. (A) WHO grade II oligodendroglioma. Isolated tumor cells (arrow) with NG2/CSPG4+ cell membranes; DAB, 400x. (B) WHO grade III oligodendroglioma. NG2/CSPG4-positive area with honeycomb appearance; DAB, OM 400x. (C) Id. Isolated CNPase-positive tumor cells in infiltration area; DAB, OM 400x. (D) Id. Sox10-positive tumour cells; DAB, OM 200x. (E) Id. NG2/CSPG4-positive reactive astrocytes inside the tumor; DAB, OM 200x. (F) Id. GFAP-positive reactive astrocytes (arrow) intermingled with GFAP-negative tumor cells; DAB, OM 400x. (G) Id. GFAP-positive (alkaline phosphatase red) and mIDH1R132H-negative reactive astrocytes (arrow) intermingled with mIDH1R132H-positive (DAB) and GFAP-negative tumor oligodendrocytes (arrow); OM 400x. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/9/6/1538) licensed under a CC-BY license.Western Blot: NG2/MCSP Antibody [NBP1-89682]
Western Blot: NG2/MCSP Antibody [NBP1-89682] - Analysis in control (vector only transfected HEK293T lysate) and CSPG4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682]
Immunocytochemistry/Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) 200x; IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM 400x; Nuclei were counterstained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/9/6/1538) licensed under a CC-BY license.Applications for NG2/MCSP Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NG2/MCSP
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Alternate Names
AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG
Entrez Gene IDs
1464 (Human)
Gene Symbol
CSPG4
UniProt
Additional NG2/MCSP Products
Product Documents for NG2/MCSP Antibody
Product Specific Notices for NG2/MCSP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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