NGEF [p Tyr87] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23339
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NGEF [p Tyr87] Antibody
Immunogen
Phospho-NGEF (Tyr-87) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 87 in mouse NGEF. This sequence has significant homology to the conserved region in human and rat NGEF. [UniProt# Q8CHT1]
Modification
p Tyr87
Specificity
The antibody detects an 80 kDa band corresponding to NGEF in Western blots of human A431 treated with pervanadate. This band is removed by alkaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for NGEF [p Tyr87] Antibody
![NGEF [p Tyr87] Antibody](https://resources.bio-techne.com/images/products/nbp3-23339_rabbit-ngef-p-tyr87-pab-199202312523640.jpg "Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] -")
Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] -
Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] - Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal NGEF (C-terminal region) and NGEF (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.![NGEF [p Tyr87] Antibody](https://resources.bio-techne.com/images/products/nbp3-23339_rabbit-ngef-p-tyr87-pab-1992023141018.jpg "Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] -")
Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] -
Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] - Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-NGEF (C-terminal region) (lanes 1 & 2) and anti-NGEF (Tyr-87) (lanes 3 & 4).Applications for NGEF [p Tyr87] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: NGEF
Alternate Names
EPHEXIN, ephexin-1, Eph-interacting exchange protein, neuronal guanine nucleotide exchange factorARHGEF27
Gene Symbol
NGEF
Additional NGEF Products
Product Documents for NGEF [p Tyr87] Antibody
Product Specific Notices for NGEF [p Tyr87] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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