NGFI-B alpha/Nur77/NR4A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56745

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Chromatin Immunoprecipitation (ChIP), IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NGFI-B alpha/Nur77/NR4A1 Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an internal portion of the human NGFI-B alpha protein (between amino acids 200-300) [UniProt P22736].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels of NGFI-B alpha/Nur77/NR4A1 protein in three independent replicates, respectively. Dot plots were used for the quantification of the expression levels of protein compared to actin control in WT and calpain-1 KO mice. Unpaired t-test in Prism 7 was used to calculate p values, *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fgene.2020.00334/full), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of NGFI-B alpha/Nur77/NR4A1 antibody (NB100-56745). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
View 3 more images

Applications for NGFI-B alpha/Nur77/NR4A1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation

1:20-1:1000. Use reported in scientific literature (Lee et al (2009))

Chromatin Immunoprecipitation (ChIP)

1:20-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Chintharlapalli et al (2005))

Immunohistochemistry

1:200. Use reported in scientific literature (PMID 31115481)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:20-1:1000. Use reported in scientific literature (Ferlini et al (2009))

Western Blot

1:500-1:1000

Reviewed Applications

Read 3 reviews rated 4.7 using NB100-56745 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis.

Long Name

Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1

Alternate Names

HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3

Entrez Gene IDs

3164 (Human); 15370 (Mouse)

Gene Symbol

NR4A1

UniProt

P22736 (Human)

Additional NGFI-B alpha/Nur77/NR4A1 Products

Product Documents for NGFI-B alpha/Nur77/NR4A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NGFI-B alpha/Nur77/NR4A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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