NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67296
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SA39-02
Concentration
1 mg/ml
Product Summary for NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
Immunogen
Synthetic peptide within Human NGFR/TNFRSF16/p75NTR aa 345-394 / 427. (SwissProt: P08138 Human; SwissProt: Q9Z0W1 Mouse; SwissProt: P07174 Rat)
Localization
Membrane
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296]
Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of p75 NGF Receptor on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Immunocytochemistry/ Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296]
Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296]
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Rat uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
Application
Recommended Usage
Flow Cytometry
1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:1000
Application Notes
IP: Use at an assay dependent concentration.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NGFR/TNFRSF16
Long Name
Nerve Growth Factor Receptor
Alternate Names
CD271, NGF R, p75 NTR, TNFRSF16
Gene Symbol
NGFR
Additional NGFR/TNFRSF16 Products
Product Documents for NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody (SA39-02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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