NHE1/SLC9A1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76848

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all NHE1/SLC9A1 Antibodies »

Product Summary for NHE1/SLC9A1 Antibody - BSA Free

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the center of the human Nhe-1. The immunogen is located within amino acids 490 - 540 of Nhe-1. Amino Acid Squence: AVKKKQETKRSINEEIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NHE1/SLC9A1 Antibody - BSA Free

Western Blot: NHE1/SLC9A1 AntibodyBSA Free [NBP1-76848]

Western Blot: NHE1/SLC9A1 AntibodyBSA Free [NBP1-76848]

Western Blot: NHE1/SLC9A1 Antibody [NBP1-76848] - MOLT4 cell lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76848]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76848]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP1-76848] - Human brain tissue using Nhe-1 antibody at 2.5 ug/ml.

Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP1-76848] -

Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP1-76848] - Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 ug/mL.

Applications for NHE1/SLC9A1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE1/SLC9A1

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

Long Name

Sodium/Hydrogen Exchanger 1

Alternate Names

APNH, SLC9A1

Gene Symbol

SLC9A1

UniProt

P19634 (Human)

Additional NHE1/SLC9A1 Products

Product Documents for NHE1/SLC9A1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NHE1/SLC9A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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