NHERF-1 Antibody (2B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00009368-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NHERF-1 Antibodies »

Product Specifications

Immunogen

SLC9A3R1 (NP_004243.1, 252 a.a. ~ 358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Specificity

SLC9A3R1 - solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (2B7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NHERF-1 Antibody (2B7)

Immunohistochemistry-Paraffin: NHERF-1 Antibody (2B7) [H00009368-M02]

Immunohistochemistry-Paraffin: NHERF-1 Antibody (2B7) [H00009368-M02]

Immunohistochemistry-Paraffin: NHERF-1 Antibody (2B7) [H00009368-M02] - Analysis of monoclonal antibody to SLC9A3R1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: NHERF-1 Antibody (2B7) [H00009368-M02]

Proximity Ligation Assay: NHERF-1 Antibody (2B7) [H00009368-M02]

Proximity Ligation Assay: NHERF-1 Antibody (2B7) [H00009368-M02] - Analysis of protein-protein interactions between PDGFRB and SLC9A3R1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:1200 and anti-SLC9A3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: NHERF-1 Antibody (2B7) [H00009368-M02] - Detection limit for recombinant GST tagged SLC9A3R1 is 0.3 ng/ml as a capture antibody.

Applications for NHERF-1 Antibody (2B7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NHERF-1

Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]

Alternate Names

EBP50NHERF-1, ezrin-radixin-moesin binding phosphoprotein-50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, NHERFNPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, sodium/hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, Solute carrier family 9 isoform A3 regulatory factor 1

Entrez Gene IDs

9368 (Human)

Gene Symbol

NHERF1

UniProt

NP_004243 (Human)

Additional NHERF-1 Products

Product Documents for NHERF-1 Antibody (2B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NHERF-1 Antibody (2B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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