NHERF-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35065

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-35065-100ul
NBP3-35065-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NHERF-1 Antibodies »

Product Summary for NHERF-1 Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 189-358 of human NHERF-1 (NP_004243.1).

Sequence:
EASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for NHERF-1 Antibody

Application
Recommended Usage

Western Blot

1:1000 - 1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NHERF-1

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Alternate Names

EBP50NHERF-1, ezrin-radixin-moesin binding phosphoprotein-50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, NHERFNPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, sodium/hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, Solute carrier family 9 isoform A3 regulatory factor 1

Gene Symbol

SLC9A3R1

Additional NHERF-1 Products

Product Documents for NHERF-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NHERF-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.