Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Nicalin Antibodies »

Product Specifications

Immunogen

Recognizes region between residues Between Between 375 and 425..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nicalin Antibody

Nicalin Antibody

Western Blot;Nicalin Antibody[NBP3-18435]

Western Blot;Nicalin Antibody Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Nicalin Antibody

Application
Recommended Usage

Immunoprecipitation

20 ug/mg lysate

Western Blot

1:10000 - 1:25000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nicalin

Nicastrin is an integral part of the Alzheimers associated gamma-secretase complex. Nicalin, a Nicastrin-like protein, is not associated with gamma-secretase but rather interacts with NOMO (Nodal modulator). Nodals are TGFbeta signaling factors that control various cell fate decisions during embryonic body planning stages in vertebrate development. The Nicalin/NOMO complex acts to regulate the Nodal signaling factors during gastrulation. This regulation most often affects the development of the axial mesoderm. Nodal signaling is an important factor for melanoma cell invasiveness and tumorigenicity and inhibition of this signal can promote melanoma cells reverting back toward a melanocyte phenotype.

Alternate Names

Ncl-1, NCLN, NET59

Gene Symbol

NCLN

Additional Nicalin Products

Product Documents for Nicalin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nicalin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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