Nicotinamide N-Methyltransferase/NNMT Antibody (2F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00004837-M03

Novus Biologicals, part of Bio-Techne
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H00004837-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nicotinamide N-Methyltransferase/NNMT Antibodies »

Product Specifications

Immunogen

NNMT (AAH00234, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

Specificity

NNMT (2F2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody (2F2)

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2. Lane 1: NNMT transfected lysatE (29.6 KDa). Lane 2: Non-transfected lysate.
ELISA: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

ELISA: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

ELISA: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.
Immunoprecipitation: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

Immunoprecipitation: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03]

Immunoprecipitation: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT transfected lysate using anti-NNMT monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NNMT rabbit polyclonal antibody.

Applications for Nicotinamide N-Methyltransferase/NNMT Antibody (2F2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nicotinamide N-Methyltransferase/NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Entrez Gene IDs

4837 (Human)

Gene Symbol

NNMT

OMIM

600008 (Human)

UniProt

AAH00234 (Human)

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody (2F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody (2F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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