Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00537
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Flow-Cytometry-NBP2-00537-img0010.jpg "Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Western-Blot-NBP2-00537-img0011.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3D8
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)
Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]
Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.![Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunocytochemistry-Immunofluorescence-NBP2-00537-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]
Immunocytochemistry/Immunofluorescence: NNMT Antibody (3D8) [NBP2-00537] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0007.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]
Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Western-Blot-NBP2-00537-img0001.jpg "Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0002.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0003.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0004.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0005.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]](https://resources.bio-techne.com/images/products/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0006.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]")
Applications for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nicotinamide N-Methyltransferase/NNMT
Long Name
Nicotinamide N-Methyltransferase
Alternate Names
Nicotinamide NMethyltransferase, NNMT
Entrez Gene IDs
4837 (Human)
Gene Symbol
NNMT
Additional Nicotinamide N-Methyltransferase/NNMT Products
Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)
Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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