Nicotinamide N-Methyltransferase/NNMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32438

Novus Biologicals, part of Bio-Techne
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NBP2-32438
NBP2-32438-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nicotinamide N-Methyltransferase/NNMT Antibodies »

Product Summary for Nicotinamide N-Methyltransferase/NNMT Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Analysis in human cell lines U2OS and SK-MEL-30 using anti-NNMT antibody. Corresponding NNMT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Immunocytochemistry/Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
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Applications for Nicotinamide N-Methyltransferase/NNMT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinamide N-Methyltransferase/NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Gene Symbol

NNMT

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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