Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81070
Recombinant Monoclonal Antibody
![Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192)Azide and BSA Free [NBP2-81070]](https://resources.bio-techne.com/images/products/Nicotinic-Acetylcholine-R-alpha-1-CHRNA1-Antibody-mAb-192-Western-Blot-NBP2-81070-img0002.jpg "Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192)Azide and BSA Free [NBP2-81070]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG2b Kappa Clone # mAb 192
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human muscle AChR.
Specificity
The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images
Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192)Azide and BSA Free [NBP2-81070]
Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Mouse skeletal muscle lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 at 0.03 ug/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa. Chimeric mouse igG1 version of mAb 192 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.![Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free [NBP2-81070]](https://resources.bio-techne.com/images/products/Nicotinic-Acetylcholine-R-alpha-1-CHRNA1-Antibody-mAb-192-Immunocytochemistry-Immunofluorescence-NBP2-81070-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free [NBP2-81070]")
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free [NBP2-81070]
Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81070, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds to the human muscle Acetylcholine receptor, which is a ligand-gated ion channel, transducing an action potential into the muscle cells. The antibody binds to the main immunogenic region (MIR) and prevents ligand-binding.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Nicotinic Acetylcholine R alpha 1/CHRNA1
Long Name
Nicotinic Acetylcholine Receptor alpha 1
Alternate Names
ACHRA, CHRNA1, CMS2A, FCCMS, nAChR alpha 1, nAchRa1, Nicotinic Acetylcholine Ra1, SCCMS
Gene Symbol
CHRNA1
Additional Nicotinic Acetylcholine R alpha 1/CHRNA1 Products
- All Products for Nicotinic Acetylcholine R alpha 1/CHRNA1
- Nicotinic Acetylcholine R alpha 1/CHRNA1 cDNA Clones
- Nicotinic Acetylcholine R alpha 1/CHRNA1 ELISA Kits
- Nicotinic Acetylcholine R alpha 1/CHRNA1 Lysates
- Nicotinic Acetylcholine R alpha 1/CHRNA1 Primary Antibodies
- Nicotinic Acetylcholine R alpha 1/CHRNA1 Proteins and Enzymes
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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