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Nidogen-1/Entactin Antibody (1G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00004811-M01

Novus Biologicals, part of Bio-Techne
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H00004811-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NID1 (NP_002499.1, 1148 a.a. ~ 1247 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIERK

Specificity

NID1 - nidogen 1 (1G3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nidogen-1/Entactin Antibody (1G3)

Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01]

Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01]

Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.Lane 1: NID1 transfected lysate(122.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Detection limit for recombinant GST tagged NID1 is 1 ng/ml as a capture antibody.

Applications for Nidogen-1/Entactin Antibody (1G3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nidogen-1/Entactin

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Alternate Names

Entactin-1, NID1, Nidogen1

Entrez Gene IDs

4811 (Human)

Gene Symbol

NID1

Additional Nidogen-1/Entactin Products

Product Documents for Nidogen-1/Entactin Antibody (1G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nidogen-1/Entactin Antibody (1G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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