Nidogen-1/Entactin Antibody (1G3)
Novus Biologicals, part of Bio-Techne | Catalog # H00004811-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NID1 (NP_002499.1, 1148 a.a. ~ 1247 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIERK
Specificity
NID1 - nidogen 1 (1G3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Nidogen-1/Entactin Antibody (1G3)
![Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01]](https://resources.bio-techne.com/images/products/Nidogen-1-Entactin-Antibody-1G3-Western-Blot-H00004811-M01-img0003.jpg "Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01]")
Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01]
Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.Lane 1: NID1 transfected lysate(122.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Detection limit for recombinant GST tagged NID1 is 1 ng/ml as a capture antibody.
Applications for Nidogen-1/Entactin Antibody (1G3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nidogen-1/Entactin
Additional Nidogen-1/Entactin Products
Product Documents for Nidogen-1/Entactin Antibody (1G3)
Product Specific Notices for Nidogen-1/Entactin Antibody (1G3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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