Nidogen-1/Entactin Antibody (ELM1) [DyLight 488]

Validated:

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Monoclonal Rat IgG2a Kappa Clone # ELM1

Please see the vial label for concentration. If unlisted please contact technical services.

Murine EHS laminin preparation

Does not react with Human.

Basement membrane

It reacts with the intact (150kDa) nidogen as well as its proteolytic fragments of 135kDa, 110kDa, 100kDa, and 80kDa. This monoclonal antibody does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Monoclonal

Rat

IgG2a Kappa

Protein A or G purified

50mM Sodium Borate

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.