NIF3L1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38295
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DFLSRNKQLYQKTEILSLEKPLLLHTGMGRLCTLDESVSLATMIDRIKRHLKLSHIRLALGVGRTLESQVKVVALCAGSGSSV
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NIF3L1 Antibody
Western Blot: NIF3L1 Antibody [NBP2-38295]
Western Blot: NIF3L1 Antibody [NBP2-38295] - Analysis in control (vector only transfected HEK293T lysate) and NIF3L1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: NIF3L1 Antibody [NBP2-38295]
Immunocytochemistry/Immunofluorescence: NIF3L1 Antibody [NBP2-38295] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.Immunohistochemistry-Paraffin: NIF3L1 Antibody [NBP2-38295]
Immunohistochemistry-Paraffin: NIF3L1 Antibody [NBP2-38295] - Staining of human tonsil shows strong cytoplasmic and nuclear positivity.Applications for NIF3L1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NIF3L1
Alternate Names
ALS2CR1, candidate 1, MDS015, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), NIF3-like protein 1, S.pombe homolog)-like 1
Gene Symbol
NIF3L1
UniProt
Additional NIF3L1 Products
Product Documents for NIF3L1 Antibody
Product Specific Notices for NIF3L1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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