NIFK Antibody (CL2237)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36750

Novus Biologicals, part of Bio-Techne
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NBP2-36750
NBP2-36750-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2237

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIFK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:IDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEI

Epitope

IDYDFPSLILQKTES

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NIFK Antibody (CL2237)

Western Blot: NIFK Antibody (CL2237) [NBP2-36750]

Western Blot: NIFK Antibody (CL2237) [NBP2-36750]

Western Blot: NIFK Antibody (CL2237) [NBP2-36750] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2237) [NBP2-36750]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2237) [NBP2-36750]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2237) [NBP2-36750] - Staining of U-2 OS cells using the Anti-MK167 monoclonal antibody, showing specific staining in the nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: NIFK Antibody (CL2237) [NBP2-36750]

Immunohistochemistry: NIFK Antibody (CL2237) [NBP2-36750]

Immunohistochemistry: NIFK Antibody (CL2237) [NBP2-36750] - Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cells.
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Applications for NIFK Antibody (CL2237)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIFK

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Alternate Names

hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67

Gene Symbol

NIFK

UniProt

Q9BYG3 (Human)

Additional NIFK Products

Product Documents for NIFK Antibody (CL2237)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NIFK Antibody (CL2237)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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