NIFK Antibody (CL2240)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36749
![Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunocytochemistry-Immunofluorescence-NBP2-36749-img0008.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL2240
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:IDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEI
Epitope
KVSGTLDTPE
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for NIFK Antibody (CL2240)
![Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunocytochemistry-Immunofluorescence-NBP2-36749-img0011.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]")
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]
Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in U251 cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0006.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human kidney shows strong nucleolar positivity in renal tubules.![Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunocytochemistry-Immunofluorescence-NBP2-36749-img0007.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]")
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]
Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in HeLa cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.![Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunocytochemistry-Immunofluorescence-NBP2-36749-img0009.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunocytochemistry-Immunofluorescence-NBP2-36749-img0010.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0001.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0002.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0003.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0004.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
![Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]](https://resources.bio-techne.com/images/products/NIFK-Antibody-CL2240-Immunohistochemistry-NBP2-36749-img0005.jpg "Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]")
Applications for NIFK Antibody (CL2240)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NIFK
Alternate Names
hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67
Gene Symbol
NIFK
UniProt
Additional NIFK Products
Product Documents for NIFK Antibody (CL2240)
Product Specific Notices for NIFK Antibody (CL2240)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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