NIPBL Antibody (3B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00025836-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Chromatin Immunoprecipitation Sequencing, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Chip Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIPBL Antibodies »

Product Specifications

Immunogen

NIPBL (AAH33847, 66 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT

Specificity

NIPBL - Nipped-B homolog (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NIPBL Antibody (3B9)

Western Blot: NIPBL Antibody (3B9) [H00025836-M01]

Western Blot: NIPBL Antibody (3B9) [H00025836-M01]

Western Blot: NIPBL Antibody (3B9) [H00025836-M01] - Detection against Immunogen (37.73 KDa) .
Immunocytochemistry/ Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01]

Immunocytochemistry/ Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01]

Immunocytochemistry/Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01] - Analysis of monoclonal antibody to NIPBL on HeLa cell . Antibody concentration 10 ug/ml.

Applications for NIPBL Antibody (3B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Use in CHIP-SEQ, IP reported in scientific literature (PMID:34315879). Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NIPBL

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

CDLS1, Delangin, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3-B, IDN3CDLS, Nipped-B homolog (Drosophila), nipped-B-like protein, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog

Entrez Gene IDs

25836 (Human)

Gene Symbol

NIPBL

OMIM

122470 (Human)

UniProt

AAH33847 (Human)

Additional NIPBL Products

Product Documents for NIPBL Antibody (3B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NIPBL Antibody (3B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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