Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all NIPBL Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1025 and 1075 of human Nipped-B-like using the numbering given in entry NP_597677.2 (GeneID 25836).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIPBL Antibody - BSA Free

Western Blot: NIPBL Antibody [NB100-93320]

Western Blot: NIPBL Antibody [NB100-93320]

Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: NIPBL Antibody [NB100-93320]

Immunoprecipitation: NIPBL Antibody [NB100-93320]

Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for NIPBL Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-6 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NIPBL

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CDLS1, Delangin, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3-B, IDN3CDLS, Nipped-B homolog (Drosophila), nipped-B-like protein, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog

Entrez Gene IDs

25836 (Human)

Gene Symbol

NIPBL

UniProt

NP_597677.2 (Human)

Additional NIPBL Products

Product Documents for NIPBL Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIPBL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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