NIPP1 Antibody (4B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005511-M05

Novus Biologicals, part of Bio-Techne
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H00005511-M05
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIPP1 Antibodies »

Product Specifications

Immunogen

PPP1R8 (NP_002704.1, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Specificity

PPP1R8 - protein phosphatase 1, regulatory (inhibitor) subunit 8 (4B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NIPP1 Antibody (4B5)

Western Blot: NIPP1 Antibody (4B5) [H00005511-M05]

Western Blot: NIPP1 Antibody (4B5) [H00005511-M05]

Western Blot: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5. Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa). Lane 2: Non-transfected lysate.
ELISA: NIPP1 Antibody (4B5) [H00005511-M05]

ELISA: NIPP1 Antibody (4B5) [H00005511-M05]

ELISA: NIPP1 Antibody (4B5) [H00005511-M05] - Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.
Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05]

Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05]

Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.

Applications for NIPP1 Antibody (4B5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NIPP1

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq]

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Alternate Names

ARD1, PPP1R8, PRO2047, RNase E

Entrez Gene IDs

5511 (Human)

Gene Symbol

PPP1R8

UniProt

NP_002704 (Human)

Additional NIPP1 Products

Product Documents for NIPP1 Antibody (4B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NIPP1 Antibody (4B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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