NIPP1 Antibody (4B5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005511-M05
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4B5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPP1R8 (NP_002704.1, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
Specificity
PPP1R8 - protein phosphatase 1, regulatory (inhibitor) subunit 8 (4B5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NIPP1 Antibody (4B5)
![Western Blot: NIPP1 Antibody (4B5) [H00005511-M05]](https://resources.bio-techne.com/images/products/NIPP1-Antibody-4B5-Western-Blot-H00005511-M05-img0003.jpg "Western Blot: NIPP1 Antibody (4B5) [H00005511-M05]")
Western Blot: NIPP1 Antibody (4B5) [H00005511-M05]
Western Blot: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5. Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa). Lane 2: Non-transfected lysate.![ELISA: NIPP1 Antibody (4B5) [H00005511-M05]](https://resources.bio-techne.com/images/products/NIPP1-Antibody-4B5-ELISA-H00005511-M05-img0001.jpg "ELISA: NIPP1 Antibody (4B5) [H00005511-M05]")
ELISA: NIPP1 Antibody (4B5) [H00005511-M05]
ELISA: NIPP1 Antibody (4B5) [H00005511-M05] - Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.![Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05]](https://resources.bio-techne.com/images/products/NIPP1-Antibody-4B5-Immunoprecipitation-H00005511-M05-img0002.jpg "Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05]")
Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05]
Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.Applications for NIPP1 Antibody (4B5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NIPP1
Long Name
Nuclear Inhibitor of Protein Phosphatase 1
Alternate Names
ARD1, PPP1R8, PRO2047, RNase E
Entrez Gene IDs
5511 (Human)
Gene Symbol
PPP1R8
UniProt
Additional NIPP1 Products
Product Documents for NIPP1 Antibody (4B5)
Product Specific Notices for NIPP1 Antibody (4B5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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