NIPP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1286

Novus Biologicals, part of Bio-Techne
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NB100-1286
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NIPP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EAWPGKKPTPSLLI corresponding to C-Terminus according to NP_002704.1, NP_054829.2, NP_612568.1.

Specificity

This antibody is expected to recognise all three human isoforms of this protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NIPP1 Antibody

Western Blot: NIPP1 Antibody [NB100-1286]

Western Blot: NIPP1 Antibody [NB100-1286]

Western Blot: NIPP1 Antibody [NB100-1286] - (1ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286] - 3.8ug/ml staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NIPP1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 2 ug/ml
Application Notes
Western blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.
IHC: In paraffin embedded Human Thymus shows speckled staining of nuclei.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NIPP1

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Alternate Names

ARD1, PPP1R8, PRO2047, RNase E

Entrez Gene IDs

5511 (Human); 100336 (Mouse)

Gene Symbol

PPP1R8

UniProt

NP_002704.1 (Human)

Additional NIPP1 Products

Product Documents for NIPP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NIPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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