NIPP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90112

Novus Biologicals, part of Bio-Techne
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NBP1-90112
NBP1-90112-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIPP1 Antibodies »

Product Summary for NIPP1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: THSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIPP1 Antibody

Western Blot: NIPP1 Antibody [NBP1-90112]

Western Blot: NIPP1 Antibody [NBP1-90112]

Western Blot: NIPP1 Antibody [NBP1-90112] - Analysis using Anti-PPP1R8 antibody NBP1-90112 (A) shows similar pattern to independent antibody NBP1-90113 (B).
Immunocytochemistry/ Immunofluorescence: NIPP1 Antibody [NBP1-90112]

Immunocytochemistry/ Immunofluorescence: NIPP1 Antibody [NBP1-90112]

Immunocytochemistry/Immunofluorescence: NIPP1 Antibody [NBP1-90112] - Staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90112]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90112]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90112] - Staining of human testis.
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Applications for NIPP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIPP1

NIPP1, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Alternate Names

ARD1, PPP1R8, PRO2047, RNase E

Gene Symbol

PPP1R8

Additional NIPP1 Products

Product Documents for NIPP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NIPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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