NIT2 Antibody (OTI2B9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02486
![Flow Cytometry: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Flow-Cytometry-NBP2-02486-img0001.jpg "Flow Cytometry: NIT2 Antibody (OTI2B9) [NBP2-02486]")
![Western Blot: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Western-Blot-NBP2-02486-img0002.jpg "Western Blot: NIT2 Antibody (OTI2B9) [NBP2-02486]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2B9
Concentration
0.74 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
30.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NIT2 Antibody (OTI2B9)
Western Blot: NIT2 Antibody (OTI2B9) [NBP2-02486]
Western Blot: NIT2 Antibody (2B9) [NBP2-02486] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.
Immunocytochemistry/Immunofluorescence: NIT2 Antibody (2B9) [NBP2-02486] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2.
![Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-02486-img0006.jpg "Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]")
Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]
Immunohistochemistry-Paraffin: NIT2 Antibody (2B9) [NBP2-02486] - Staining of paraffin-embedded pancreas tissue using anti-NIT2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-02486-img0003.jpg "Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]")
![Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-02486-img0004.jpg "Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]")
![Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]](https://resources.bio-techne.com/images/products/NIT2-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-02486-img0005.jpg "Immunohistochemistry-Paraffin: NIT2 Antibody (OTI2B9) [NBP2-02486]")
Applications for NIT2 Antibody (OTI2B9)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.74 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NIT2
Alternate Names
EC 3.5.1.3, MGC111199, Nit protein 2, nitrilase family, member 2, Nitrilase homolog 2, omega-amidase NIT2
Entrez Gene IDs
56954 (Human)
Gene Symbol
NIT2
Additional NIT2 Products
Product Documents for NIT2 Antibody (OTI2B9)
Product Specific Notices for NIT2 Antibody (OTI2B9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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