Nitrilase 1 Antibody (OTI3A11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02462

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A11

Concentration

0.87 mg/ml

View all Nitrilase 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nitrilase 1 Antibody (OTI3A11)

Western Blot: Nitrilase 1 Antibody (OTI3A11) [NBP2-02462]

Western Blot: Nitrilase 1 Antibody (OTI3A11) [NBP2-02462]

Western Blot: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nitrilase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nitrilase 1.
Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (3A11) [NBP2-02462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nitrilase 1.
Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (OTI3A11) [NBP2-02462]

Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (OTI3A11) [NBP2-02462]

Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Staining of paraffin-embedded pancreas tissue using anti-Nitrilase 1 mouse monoclonal antibody.
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Applications for Nitrilase 1 Antibody (OTI3A11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.87 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nitrilase 1

Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2

Alternate Names

EC 3.5, MGC57670, nitrilase 1, nitrilase homolog 1

Entrez Gene IDs

4817 (Human)

Gene Symbol

NIT1

Additional Nitrilase 1 Products

Product Documents for Nitrilase 1 Antibody (OTI3A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nitrilase 1 Antibody (OTI3A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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